The Severe Acute Respiratory Syndrome Coronavirus-2, or COVID, was announced by WHO on February 11, 2020. Examination of SARS-CoV2 by qRT-PCR) method in Laboratorium Kesehatan Daerah Kabupaten Sukabumi (LABKESDA) is one of the health service support units in the Sukabumi area. The number of PCR examination requests is increasing every day, whereas, at this stage, the temperature should reach 90°C as recommended, which can take 20-25 minutes. Sometimes Medical Laboratory Technologist proceed the specimens at 60°C-90°C. Given the suggested temperature at the time of extraction, here, the researchers wanted to prove whether below 90 degrees affected the virus extraction process. This study aimed to determine the effect of differences in extraction temperature on the SARS-CoV2 q-PCR examination process on the ct value of patient specimens. This study was an experimental study with difference temperatures, including 60°C, 80°C, and 90°C. The population of this study were 30 patients at LABKESDA Kabupaten Sukabumi. A one-way ANOVA test analysis was used with software IBM SPSS 23.0. The result showed no significant different in the ct value among all difference temperatures on the SARS-CoV2 q-PCR examination with a p-value of more than 0.05 (p=0.785). So it can be concluded that the difference in extraction temperature does not affect the result of the CT value.