Evaluating Mineral and Herbomineral formulations using advanced analytical tests helps to standardize Ayurveda pharmaceutics. This study focused on what changes happened during and after the preparation of Hinguleshwar rasa and cinnabar on the elemental PPM level. ICPMS was carried out for elemental qualitative analysis of Raw Hingul, Shodhit Hingul, Ashodhit Vatsanabha, Shodhit Vatsanabha, Pippali, and Hinguleshwar Rasa. In this study in the sample of Shodhit Hingul (purified Cinnabar), we found elements like Chromium (CR), Sodium (NA), and Tungsten (W). but these elements were not present in the sample of unpurified raw Hingul. In the sample of Shodhit and Ashodhit Vatsanabha (purified & unpurified Aconitum ferox), we did not find any different significant element in this test. In the sample of Hinguleshwar Rasa, we did not find elements that are present in Shodhit Hingul (purified Cinnabar) & Shodhit Vatsanabha (purified Aconitum ferox) like Chromium (CR), Gallium (GA), Molybdenum (MO), Nickel (NI), Lead (PB), Tungsten (W). XRD study of sample raw Hingul, Shodhit Hingul, and Hinguleshwar Rasa showed that the intensity of peak was increased but the atomic structure of Hingul was not affected in all the samples. FEG SEM helped to get images of all the samples under magnification of x 10,000 and x 50,000. In the ICP MS Isotopic evaluation of mercury got isotopic changes in naturally occurring stable Isotopes of Mercury Hg 196, Hg199, Hg200, Hg201, Hg202, and Hg204 in the raw, purified Cinnabar and Hinguleshwar Rasa. 

Hence this study concludes that advanced analytical tests may help to standardize drug and formulation into elemental levels in Ayurveda pharmaceutics.

Dr. Ajay B. Sonawane is an Ayurveda consultant and physician at Kalapana Ayurveda Hospital. He won the International Research Vibhushan Award in August 2021, the Young Researcher Award in March 2023, the Best Ph.D. Research Award in November 2023 in Nepal, and Three Best National Scientific Oral Paper Presentation Awards in the National conferences.