Tea Tree Oil (TTO), obtained from tea tree (Melaleuca alternifolia), possesses many properties, with its anti-inflammatory activity being particularly noteworthy. TTO formulations in gel were made from the pharmacotechnical and pharmacopeial information, from which one was chosen to make the formulation with the two concentrations of TTO from which one was chosen to make the formulation with the two concentrations of TTO. A 5% dilution of croton oil. Five groups of 6 mice each were formed. A 5% (v/v) solution of croton oil in acetone was used in the right ear cause edema and evaluate the anti-inflammatoy activity. The results of this study show that TTO is anti-inflammatory activity in vivo test conducted in animals.