Salar Saadi Hussain Dizayee
Hawler Medical University, IraqPresentation Title:
The pathological significance of HRAS gene mutation in colorectal cancer patients in Erbil province
Abstract
Background and objective: RAS gene mutations, including Harvey RAS, are a biomarker in studying many types of cancer, including colorectal cancer. Studying the molecular and mutations of the HRAS gene help in understanding the nature of the tumor and helps to formulate a suitable plan for prevention and treatment. The aim of the present study is to identify HRAS mutations among a sample of colorectal cancer.
Methods: The sample for the present study involved 20 Formalin fixed embedded tissue of colorectal carcinoma and 10 adjacent normal tissues were obtained from Rizgary Teaching Hospital in Erbil. Genomic DNA was isolated from 10 µm-thick paraffin-embedded tissue sections. Easy Thyroid kit to detect HRAS mutations in codons 12, 13, and 61. The HRAS mutations were based on the detection of at least 1% of tumor cells and were provided by the manufacturer of the kit. The Ct cut-off values were as follows for the different mutations: codon 12,13 Ct >35, codon 61 Ct 33.
Results: The sample of the present study involved 20 patients (60% males and 40% females) with CRC. The mean age was 54.20 years, with an SD ±19.80. The study found a single HRAS mutation in the sample study. The size of the tumor mutated by HRAS is significantly different than other tumors (P= 0.009).
Conclusion: there was an HRAS mutation in a sample of colorectal cancers and the size of the tumor was different than other tumors in the study sample. Further studies needed about the mutation of the HRAS gene.
Biography
Salar Saadi has completed her MSc at the age of 27 years from Duhok University, Iraq, and now she is a PhD students in immunogenetic specialty. She is the member of teaching staff at Nursing college in Haler Medical University. She has 7 publications ,She has been serving as an editorial assistant member of EJNM journal.
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